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apai cut site  (Thermo Fisher)


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    Thermo Fisher apai cut site
    a Fragment sizes predicted by <t>ApaI</t> digestion in <t>‘Nantong-xiaofangshi’,</t> <t>Diospyros</t> lotus and ‘Kanshu’. b Polyacrylamide gel electrophoresis of restrictive digestion of different RT-PCR products. c Samples from an 82 cm tall grafted tree with ‘Kanshu’ as scion, ‘Nantong-xiaofangshi’ as interstock and Diospyros lotus as rootstock. d CAPS analysis on purified RT-PCR products of different tissues in ‘Kanshu’/‘Nantong-xiaofangshi’/ Diospyros lotus . e Comparison of total DkGA2ox1 / DLGA2ox1 / KSGA2ox1 transcripts in ‘Kanshu’/‘Nantong-xiaofangshi’/ Diospyros lotus . DkGA2ox1 , DLGA2ox1 and KSGA2ox1 are the sequences in ‘Nantong-xiaofangshi’, Diospyros lotus and ‘Kanshu’, respectively; N.x: ‘Nantong-xiaofangshi’ / Diospyros lotus , DL: Diospyros lotus , KS: ‘Kanshu’, M’: mixture of cDNA from N.x, DL and KS2, KS1: ‘Kanshu’ /‘Nantong-xiaofangshi’/ Diospyros lotus , KS2: ‘Kanshu’ / Diospyros lotus , GU1: graft union of Diospyros lotus and ‘Nantong-xiaofangshi’, IN: ‘Nantong-xiaofangshi’ interstock, GU2: graft union of ‘Nantong-xiaofangshi’ and ‘Kanshu’, 1-3: ‘Kanshu’ scion every 10 cm from the GU2, 4: young leaves at the top of the scion. M: DNA ladder marker 500. Scale bars = 5 cm. Note: double asterisk (**) indicates a significant difference ( P < 0.01). The purified PCR products were diluted to the same concentration for enzyme cutting
    Apai Cut Site, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apai+cut+site/apai+cut+site/pmc06491603-166-16-21
    Average 90 stars, based on 1 article reviews
    apai cut site - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Transcriptomic analysis of interstock-induced dwarfism in Sweet Persimmon ( Diospyros kaki Thunb.)"

    Article Title: Transcriptomic analysis of interstock-induced dwarfism in Sweet Persimmon ( Diospyros kaki Thunb.)

    Journal: Horticulture Research

    doi: 10.1038/s41438-019-0133-7

    a Fragment sizes predicted by ApaI digestion in ‘Nantong-xiaofangshi’, Diospyros lotus and ‘Kanshu’. b Polyacrylamide gel electrophoresis of restrictive digestion of different RT-PCR products. c Samples from an 82 cm tall grafted tree with ‘Kanshu’ as scion, ‘Nantong-xiaofangshi’ as interstock and Diospyros lotus as rootstock. d CAPS analysis on purified RT-PCR products of different tissues in ‘Kanshu’/‘Nantong-xiaofangshi’/ Diospyros lotus . e Comparison of total DkGA2ox1 / DLGA2ox1 / KSGA2ox1 transcripts in ‘Kanshu’/‘Nantong-xiaofangshi’/ Diospyros lotus . DkGA2ox1 , DLGA2ox1 and KSGA2ox1 are the sequences in ‘Nantong-xiaofangshi’, Diospyros lotus and ‘Kanshu’, respectively; N.x: ‘Nantong-xiaofangshi’ / Diospyros lotus , DL: Diospyros lotus , KS: ‘Kanshu’, M’: mixture of cDNA from N.x, DL and KS2, KS1: ‘Kanshu’ /‘Nantong-xiaofangshi’/ Diospyros lotus , KS2: ‘Kanshu’ / Diospyros lotus , GU1: graft union of Diospyros lotus and ‘Nantong-xiaofangshi’, IN: ‘Nantong-xiaofangshi’ interstock, GU2: graft union of ‘Nantong-xiaofangshi’ and ‘Kanshu’, 1-3: ‘Kanshu’ scion every 10 cm from the GU2, 4: young leaves at the top of the scion. M: DNA ladder marker 500. Scale bars = 5 cm. Note: double asterisk (**) indicates a significant difference ( P < 0.01). The purified PCR products were diluted to the same concentration for enzyme cutting
    Figure Legend Snippet: a Fragment sizes predicted by ApaI digestion in ‘Nantong-xiaofangshi’, Diospyros lotus and ‘Kanshu’. b Polyacrylamide gel electrophoresis of restrictive digestion of different RT-PCR products. c Samples from an 82 cm tall grafted tree with ‘Kanshu’ as scion, ‘Nantong-xiaofangshi’ as interstock and Diospyros lotus as rootstock. d CAPS analysis on purified RT-PCR products of different tissues in ‘Kanshu’/‘Nantong-xiaofangshi’/ Diospyros lotus . e Comparison of total DkGA2ox1 / DLGA2ox1 / KSGA2ox1 transcripts in ‘Kanshu’/‘Nantong-xiaofangshi’/ Diospyros lotus . DkGA2ox1 , DLGA2ox1 and KSGA2ox1 are the sequences in ‘Nantong-xiaofangshi’, Diospyros lotus and ‘Kanshu’, respectively; N.x: ‘Nantong-xiaofangshi’ / Diospyros lotus , DL: Diospyros lotus , KS: ‘Kanshu’, M’: mixture of cDNA from N.x, DL and KS2, KS1: ‘Kanshu’ /‘Nantong-xiaofangshi’/ Diospyros lotus , KS2: ‘Kanshu’ / Diospyros lotus , GU1: graft union of Diospyros lotus and ‘Nantong-xiaofangshi’, IN: ‘Nantong-xiaofangshi’ interstock, GU2: graft union of ‘Nantong-xiaofangshi’ and ‘Kanshu’, 1-3: ‘Kanshu’ scion every 10 cm from the GU2, 4: young leaves at the top of the scion. M: DNA ladder marker 500. Scale bars = 5 cm. Note: double asterisk (**) indicates a significant difference ( P < 0.01). The purified PCR products were diluted to the same concentration for enzyme cutting

    Techniques Used: Polyacrylamide Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Purification, Comparison, Marker, Concentration Assay

    Related Articles

    Construct:

    Article Title: Transcriptomic analysis of interstock-induced dwarfism in Sweet Persimmon ( Diospyros kaki Thunb.)
    Article Snippet: Amplified cDNA sequences were purified and subcloned into the pEASY ® - Blunt cloning vector (TransGen Biotech, Beijing, China) for sequencing. .. Restriction enzyme cleavage sites of Diospyros lotus , N.x, and ‘Kanshu’ were constructed, and a specific ApaI cut site was identified (Thermo Scientific, Waltham, MA, USA). .. RT-PCR products were amplified with the DkGA2ox1 -S-F1 and DkGA2ox1 -S-R1 primers (Table ) according to the instructions of PrimerSTAR GXL Polymerase (Takara, Dalian, China) and using the following PCR conditions: initial denaturation at 98 °C for 5 min, followed by 30 cycles of 98 °C for 10 s, 60 °C for 15 s and 68 °C for 30 s, and a final extension at 68 °C for 10 min.



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    apai cut site  (Thermo Fisher)
    90
    Thermo Fisher apai cut site
    a Fragment sizes predicted by <t>ApaI</t> digestion in <t>‘Nantong-xiaofangshi’,</t> <t>Diospyros</t> lotus and ‘Kanshu’. b Polyacrylamide gel electrophoresis of restrictive digestion of different RT-PCR products. c Samples from an 82 cm tall grafted tree with ‘Kanshu’ as scion, ‘Nantong-xiaofangshi’ as interstock and Diospyros lotus as rootstock. d CAPS analysis on purified RT-PCR products of different tissues in ‘Kanshu’/‘Nantong-xiaofangshi’/ Diospyros lotus . e Comparison of total DkGA2ox1 / DLGA2ox1 / KSGA2ox1 transcripts in ‘Kanshu’/‘Nantong-xiaofangshi’/ Diospyros lotus . DkGA2ox1 , DLGA2ox1 and KSGA2ox1 are the sequences in ‘Nantong-xiaofangshi’, Diospyros lotus and ‘Kanshu’, respectively; N.x: ‘Nantong-xiaofangshi’ / Diospyros lotus , DL: Diospyros lotus , KS: ‘Kanshu’, M’: mixture of cDNA from N.x, DL and KS2, KS1: ‘Kanshu’ /‘Nantong-xiaofangshi’/ Diospyros lotus , KS2: ‘Kanshu’ / Diospyros lotus , GU1: graft union of Diospyros lotus and ‘Nantong-xiaofangshi’, IN: ‘Nantong-xiaofangshi’ interstock, GU2: graft union of ‘Nantong-xiaofangshi’ and ‘Kanshu’, 1-3: ‘Kanshu’ scion every 10 cm from the GU2, 4: young leaves at the top of the scion. M: DNA ladder marker 500. Scale bars = 5 cm. Note: double asterisk (**) indicates a significant difference ( P < 0.01). The purified PCR products were diluted to the same concentration for enzyme cutting
    Apai Cut Site, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apai+cut+site/apai+cut+site/pmc06491603-166-16-21
    Average 90 stars, based on 1 article reviews
    apai cut site - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    a Fragment sizes predicted by ApaI digestion in ‘Nantong-xiaofangshi’, Diospyros lotus and ‘Kanshu’. b Polyacrylamide gel electrophoresis of restrictive digestion of different RT-PCR products. c Samples from an 82 cm tall grafted tree with ‘Kanshu’ as scion, ‘Nantong-xiaofangshi’ as interstock and Diospyros lotus as rootstock. d CAPS analysis on purified RT-PCR products of different tissues in ‘Kanshu’/‘Nantong-xiaofangshi’/ Diospyros lotus . e Comparison of total DkGA2ox1 / DLGA2ox1 / KSGA2ox1 transcripts in ‘Kanshu’/‘Nantong-xiaofangshi’/ Diospyros lotus . DkGA2ox1 , DLGA2ox1 and KSGA2ox1 are the sequences in ‘Nantong-xiaofangshi’, Diospyros lotus and ‘Kanshu’, respectively; N.x: ‘Nantong-xiaofangshi’ / Diospyros lotus , DL: Diospyros lotus , KS: ‘Kanshu’, M’: mixture of cDNA from N.x, DL and KS2, KS1: ‘Kanshu’ /‘Nantong-xiaofangshi’/ Diospyros lotus , KS2: ‘Kanshu’ / Diospyros lotus , GU1: graft union of Diospyros lotus and ‘Nantong-xiaofangshi’, IN: ‘Nantong-xiaofangshi’ interstock, GU2: graft union of ‘Nantong-xiaofangshi’ and ‘Kanshu’, 1-3: ‘Kanshu’ scion every 10 cm from the GU2, 4: young leaves at the top of the scion. M: DNA ladder marker 500. Scale bars = 5 cm. Note: double asterisk (**) indicates a significant difference ( P < 0.01). The purified PCR products were diluted to the same concentration for enzyme cutting

    Journal: Horticulture Research

    Article Title: Transcriptomic analysis of interstock-induced dwarfism in Sweet Persimmon ( Diospyros kaki Thunb.)

    doi: 10.1038/s41438-019-0133-7

    Figure Lengend Snippet: a Fragment sizes predicted by ApaI digestion in ‘Nantong-xiaofangshi’, Diospyros lotus and ‘Kanshu’. b Polyacrylamide gel electrophoresis of restrictive digestion of different RT-PCR products. c Samples from an 82 cm tall grafted tree with ‘Kanshu’ as scion, ‘Nantong-xiaofangshi’ as interstock and Diospyros lotus as rootstock. d CAPS analysis on purified RT-PCR products of different tissues in ‘Kanshu’/‘Nantong-xiaofangshi’/ Diospyros lotus . e Comparison of total DkGA2ox1 / DLGA2ox1 / KSGA2ox1 transcripts in ‘Kanshu’/‘Nantong-xiaofangshi’/ Diospyros lotus . DkGA2ox1 , DLGA2ox1 and KSGA2ox1 are the sequences in ‘Nantong-xiaofangshi’, Diospyros lotus and ‘Kanshu’, respectively; N.x: ‘Nantong-xiaofangshi’ / Diospyros lotus , DL: Diospyros lotus , KS: ‘Kanshu’, M’: mixture of cDNA from N.x, DL and KS2, KS1: ‘Kanshu’ /‘Nantong-xiaofangshi’/ Diospyros lotus , KS2: ‘Kanshu’ / Diospyros lotus , GU1: graft union of Diospyros lotus and ‘Nantong-xiaofangshi’, IN: ‘Nantong-xiaofangshi’ interstock, GU2: graft union of ‘Nantong-xiaofangshi’ and ‘Kanshu’, 1-3: ‘Kanshu’ scion every 10 cm from the GU2, 4: young leaves at the top of the scion. M: DNA ladder marker 500. Scale bars = 5 cm. Note: double asterisk (**) indicates a significant difference ( P < 0.01). The purified PCR products were diluted to the same concentration for enzyme cutting

    Article Snippet: Restriction enzyme cleavage sites of Diospyros lotus , N.x, and ‘Kanshu’ were constructed, and a specific ApaI cut site was identified (Thermo Scientific, Waltham, MA, USA).

    Techniques: Polyacrylamide Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Purification, Comparison, Marker, Concentration Assay